ELISA
About ELISA
Enzyme-Linked Immunosorbent Assay (ELISA) is the assay format that is very popular in terms of efficiency, reproducibility, performance and validation in the plant pathogen diagnostic.
Our ELISA reagents are delivered with Lot specific data sheets . We are committed to lot-to-lot consistency for increased test security.
Individual ELISA reagents
| IgG and Conjugate are available for 100, 500 and for 1000 assays. |
| Reagent | Assays | |||
| 100 | 500 | 1000 | 5000* | |
| IgG | 0.025 ml | 0.1 ml | 0.2 ml | 1 ml |
| Conjugate | 0.025 ml | 0.1 ml | 0.2 ml | 1 ml |
|
*For the six main potato viruses (PLRV, PVA, PVM, PVS, PVX and PVY),
reagents are also available for 5000 assays.
|
| Control | Assays | |||
| 12 | ||||
| Positive control | 2.5 ml | |||
| Negative control | 2.5 ml |
ELISA Reagent Sets
| Component | Assays | |||
| 480 | 960 | 5000* | ||
| IgG | 0.1 ml | 0.2 ml | 1 ml | |
| Conjugate | 0.1 ml | 0.2 ml | 1 ml | |
| Positive control | 2.5 ml | 2.5 ml | 5 x 2.5 ml | |
| Negative control | 2.5 ml | 2.5 ml | 5 x 2.5 ml | |
| Microtiter plates | 5 | 10 | 60 |
|
*For the six main potato viruses (PLRV, PVA, PVM, PVS, PVX and PVY),
reagents are also available for 5000 assays.
|
ELISA Complete Kits
| Component | Assays | |||
| 96 | 480 | 960 | 5000* | |
| IgG | 25 ul | 0.1 ml | 0.2 ml | 1 ml |
| Conjugate | 25 ul | 0.1 ml | 0.2 ml | 1 ml |
| Positive control | 2.5 ml | 2.5 ml | 2.5 ml | 1 ml |
| Negative control | 2.5 ml | 2.5 ml | 2.5 ml | 5 x 2.5 ml |
|
Extraction buffer (10x)
|
50 ml | 250 ml | 500 ml | 5 x 500 ml |
| Coating buffer | 1 tablet | 1 tablet | 2 tablets | 10 tablets |
|
Conjugate buffer (10x)
|
2 ml | 10 ml | 20 ml | 100 ml |
|
Substrate buffer (5x)
|
4 ml | 20 ml | 2 x 20 ml | 200 ml |
| Washing buffer | 1 tablet | 1 pouch | 2 pouches | 2 containers |
|
Substrate (pNPP)
|
4 tablets
(5 mg)
|
5 tablets
(20 mg)
|
10 tablets
(20 mg)
|
bulk powder
(5g)
|
| Egg Albumin | 5 g | 25 g | 50 g | 250 g |
| Microtiter plates | 1 | 5 | 10 | 60 |
|
*For the six main potato viruses (PLRV, PVA, PVM, PVS, PVX and PVY),
reagents are also available for 5000 assays.
|
Available ELISA Tests
| Crop | Download pdf | |
| Potatoes | Pathogens_of_potatoes.pdf | |
| Grapevines | Pathogens_of_grapevines.pdf | |
| Vegetables | Pathogens_of_vegetables.pdf | |
| Fruit Trees & Small Fruits | Pathogens_of_fruittrees.pdf | |
| Field Crops | Pathogens_of_fieldcrops.pdf | |
| Ornamentals | Pathogens_of_ornamentals.pdf | |