BIOREBA Testing Service Lab
for plant pathogen diagnostics
BIOREBA Testing Service provides you its expertise in diagnostic assays for the detection of plant pathogens (viruses, bacteria, fungi and phytoplasma).
More than 30 years of experience with analysis of samples of most agricultural sectors and employment of different state-of-the-art methods and technologies guarantee the highest possible accuracy of test results.
Each case is treated in close communication with the customer to determine the optimal sampling and transport of the samples, as well as the arrangement of the most appropriate testing method and terms. This to make sure each customer gets the right service for its needs in the most cost effective and rapid way.
Our testing service is in accordance to the strict rules and quality standards according to ISO/IEC 17025 and confidentiality is maintained throughout communications and data processing.
Sending Samples, Prices, Testing Methods



Sending Samples
2) We are providing special extraction bags for leaf samples. An import permit is required for customers outside the European Union and for certain type of samples (e.g. tomato seeds, tubers, bulbs).
3) Leaf samples 1: Take approx. 0.50 g of plant material. A piece of 4 x 4 cm or several fragments are generally recommended. Place the sample in the rear compartment of the extraction bag. Label the bag with the number corresponding to the sample.
4) Record your samples in a list consecutively numbered and send us this list electronically by email (e.g. Excel sheet).
5) Send the samples with priority shipping. Avoid shipping during week-ends or holidays. If required, attach a copy of the import permit issued by the Swiss Federal Office of Agriculture precedingly provided by BIOREBA by email or fax.
6) You will receive the written testing service report according to agreement or within 5-10 working days after receipt of your samples.
Prices
Please contact us by phone (+41 61 712 11 25) or email ([email protected]) for an offer. Our prices depend on:
- Quantity of samples
- Sample types
- Number of pathogens to be tested per sample
- Testing method
Our basic fee per order is CHF 150 or EUR 150.
Testing Methods
The serological method DAS-ELISA (double antibody sandwich enzyme-linked immunosorbant assay) is primarily used for the analysis of plant samples for the presence of specific pathogens. This method is economic and suitable in most cases and is based on the same BIOREBAs validated reagents that are used by our customers world-wide.
We also offer the analysis of your samples with the molecular biological method polymerase chain reaction (classical or real-time PCR). Please check our available tests for your crop of interest.
Potato Tests



PLRV Potato leafroll virus (samples: leaves, tubers, sprouts)
PVA Potato virus A (samples: leaves, tubers, sprouts)
PVM Potato virus M (samples: leaves, tubers, sprouts)
PVS Potato virus S (samples: leaves, tubers, sprouts)
PVX Potato virus X (samples: leaves, tubers, sprouts)
PVY Potato virus Y (samples: leaves, tubers, sprouts)
AMV Alfalfa mosaic virus (samples: leaves, tubers, sprouts)
APMoV Andean potato mottle virus (samples: leaves, tubers, sprouts)
PMTV Potato mop-top virus (samples: leaves, tubers, sprouts)
PVV Potato virus V (samples: leaves, tubers, sprouts)
Rs Ralstonia solanacearum (samples: tubers, sprouts, stem base)
Sss Spongospora subterranea f. sp. Subterranea (samples: tubers, soil and peat substrates. Please note: Tests of soil and peat substrates is not included in the range of accredited services.)1
TBRV Tomato black ring virus–potato strain (samples: leaves, tubers, sprouts)
TNV Tobacco necrosis virus (samples: leaves, tubers, sprouts)
TSWV Tomato spotted wilt virus (samples: leaves, tubers, sprouts)
Verticillium Verticillium spp. (samples: stem base)
Our PCR Tests for Potato samples
Dsp Dickeya spp.2, Patr Pectobacterium atrosepticum2, Pwas Pectobacterium wasabiae2 & Pcbr Pectobacterium carotovorum subsp. brasiliense2 (samples: 6 x 50 tubers)
PVA Potato virus A (samples: leaves, tubers, sprouts)
PVM Potato virus M (samples: leaves, tubers, sprouts)
PVS Potato virus S (samples: leaves, tubers, sprouts)
PVX Potato virus X (samples: leaves, tubers, sprouts)
PVY Potato virus Y (samples: leaves, tubers, sprouts)
Grapevine Tests



However, the virus concentration varies considerably according to tissue source, virus and
physiological state of the host in function of time of the season. These facts have to be
considered for obtaining reliable results.
Basically, all viruses can be detected in mature canes during dormancy. When testing
leaves, however, viruses are separated into two distinct groups having each their specific
requirements. Under climatic conditions of Switzerland and Middle Europe, following
recommendations for tissue sampling can be given:
in the season as well as bark (phloem) scrapings from mature canes during dormancy.
Phytoplasma (Flavescence dorée, Bois noir) are best detectable in veins and petioles of
well-developed mature leaves late in the growing season, as for viruses of group B. During
the rest of the year, phytoplasma are preferably detected in root tissue.
Vegetable Tests



Fruit Tree & Small Fruit Tests



Field Crop Tests



Ornamental Tests






