BIOREBA Testing Service Lab

for plant pathogen diagnostics

 
 
                     

BIOREBA Testing Service provides you its expertise in diagnostic assays for the detection of plant pathogens (viruses, bacteria, fungi and phytoplasma).

More than 30 years of experience with analysis of samples of most agricultural sectors and employment of different state-of-the-art methods and technologies guarantee the highest possible accuracy of test results.

Each case is treated in close communication with the customer to determine the optimal sampling and transport of the samples, as well as the arrangement of the most appropriate testing method and terms. This to make sure each customer gets the right service for its needs in the most cost effective and rapid way.

Our testing service is in accordance to the strict rules and quality standards according to ISO/IEC 17025 and confidentiality is maintained throughout communications and data processing.



Sending Samples, Prices, Testing Methods

 


Sending Samples

 
1) Please contact us by phone (+41 61 712 11 25) or email ([email protected]) to specify kind of samples and test parameters, and to arrange conditions and timing.

2) We are providing special extraction bags for leaf samples. An import permit is required for customers outside the European Union and for certain type of samples (e.g. tomato seeds, tubers, bulbs).
For the timing and selection of the appropriate test sample, it is important to bear in mind that the concentration of pathogens may vary according to season, plant tissue, meteorological conditions, variety of host plant and kind of pathogen. Furthermore, pathogen distribution can be systemic or locally restricted; hence, the appropriate and representative sampling is one of the most crucial factors to get reliable diagnostic results. We recommend to arrange the modality of sampling with us to ascertain that tests can be carried out under optimal conditions.

3) Leaf samples 1: Take approx. 0.50 g of plant material. A piece of 4 x 4 cm or several fragments are generally recommended. Place the sample in the rear compartment of the extraction bag. Label the bag with the number corresponding to the sample.
Tubers 1: Please ship tubers washed and dried (not wet) in a bag permeable to air.
Wood samples 1: Cut canes or branches of 1-3 years old wood of 30-50 cm in length (or at least 2-3 internodes). Do not use dry (dead) wood samples. Label the samples with self-adhesive paper, or another appropriate permanent label according to your sample list.

4) Record your samples in a list consecutively numbered and send us this list electronically by email (e.g. Excel sheet).

5) Send the samples with priority shipping.  Avoid shipping during week-ends or holidays. If required, attach a copy of the import permit issued by the Swiss Federal Office of Agriculture precedingly provided by BIOREBA by email or fax.

6) You will receive the written testing service report according to agreement or within 5-10 working days after receipt of your samples.
 
 

 

Prices

Please contact us by phone (+41 61 712 11 25) or email ([email protected]) for an offer. Our prices depend on:

  • Quantity of samples
  • Sample types
  • Number of pathogens to be tested per sample
  • Testing method

Our basic fee per order is CHF 150 or EUR 150.

 

Testing Methods

The serological method DAS-ELISA (double antibody sandwich enzyme-linked immunosorbant assay) is primarily used for the analysis of plant samples for the presence of specific pathogens. This method is economic and suitable in most cases and is based on the same BIOREBAs validated reagents that are used by our customers world-wide.

We also offer the analysis of your samples with the molecular biological method polymerase chain reaction (classical or real-time PCR). Please check our available tests for your crop of interest.

 

Potato Tests

 

 
Our ELISA Tests for Potato samples
PLRV Potato leafroll virus (samples: leaves, tubers, sprouts)
PVA Potato virus A (samples: leaves, tubers, sprouts)
PVM Potato virus M (samples: leaves, tubers, sprouts)
PVS Potato virus S (samples: leaves, tubers, sprouts)
PVX Potato virus X (samples: leaves, tubers, sprouts)
PVY Potato virus Y (samples: leaves, tubers, sprouts)
AMV Alfalfa mosaic virus (samples: leaves, tubers, sprouts)
APLV Andean potato latent virus (samples: leaves, tubers, sprouts)
APMMV Andean potato mild mosaic virus (samples: leaves, tubers, sprouts) / NOTE: detected with APLV reagent
APMoV Andean potato mottle virus (samples: leaves, tubers, sprouts)
PMTV Potato mop-top virus (samples: leaves, tubers, sprouts)
PVV Potato virus V (samples: leaves, tubers, sprouts)
PYBV Potato yellow blotch virus (samples: leaves, tubers, sprouts) / NOTE: detected with the PVA reagent
Rs Ralstonia solanacearum (samples: tubers, sprouts, stem base)
Sss Spongospora subterranea f. sp. Subterranea (samples: tubers, soil and peat substrates. Please note: Tests of soil and peat substrates is not included in the range of accredited services.)1
TBRV Tomato black ring virus–potato strain (samples: leaves, tubers, sprouts)
TNV Tobacco necrosis virus (samples: leaves, tubers, sprouts)
TSWV Tomato spotted wilt virus (samples: leaves, tubers, sprouts)
Verticillium Verticillium spp. (samples: stem base)
1Tests of soil and peat substrates are not included in the range of accredited services.

Our PCR Tests for Potato samples
Cms Clavibacter michiganensis subsp. sepedonicus (tubers)
Rs Ralstonia solanacearum (tubers, stem base)
Lso Candidatus Liberibacter solanacearum (tubers)
Pcc Pectobacterium carotovorum subsp. carotovorum(tubers, stem base)
 
Package Black leg disease
Dsp Dickeya spp.2Patr Pectobacterium atrosepticum2Pwas Pectobacterium wasabiae2 & Pcbr Pectobacterium carotovorum subsp. brasiliense2 (samples: 6 x 50 tubers)
 
2PCR-Tests of Pcc, Dsp, Patr, Pwas und Pcbr are not included in the range of accredited services.
 
PLRV Potato leafroll virus (samples: leaves, tubers, sprouts)
PVA Potato virus A (samples: leaves, tubers, sprouts)
PVM Potato virus M (samples: leaves, tubers, sprouts)
PVS Potato virus S (samples: leaves, tubers, sprouts)
PVX Potato virus X (samples: leaves, tubers, sprouts)
PVY Potato virus Y (samples: leaves, tubers, sprouts)
PMTV Potato mop-top virus (samples: leaves, tubers, sprouts)

Grapevine Tests

 
 
 
Our ELISA Tests for Grapevine samples
Group A
ArMV Arabis mosaic virus (samples: leaves1, canes3)
GFLV Grapevine fanleaf virus leaves1, canes3
ArMV+GFLV Arabis mosaic virus + Grapevine fanleaf virus (samples: leaves1, canes3)
GPGV Grapevine pinot gris virus (samples: leaves1canes3)
RpRSV-ch Raspberry ringspot virus-ch (samples: leaves1, canes3)
RpRSV-g Raspberry ringspot virus-g (samples: leaves1, canes3)
TBRV Tomato black ring virus (samples: leaves1, canes3)
TRSV Tobacco ringspot virus (samples: leaves1, canes3)
ToRSV Tomato ringspot virus (samples: leaves1, canes3)
ToRSV-Ch Tomato ringspot virus-Ch (samples: leaves1, canes3)
SLRSV Strawberry latent ringspot virus (samples: leaves1, canes3)
 
Group B
GLRaV-1 Grapevine leafroll-assoc. virus 1 (samples: leaves1, petioles2, canes3)
GLRaV-1+3 Grapevine leafroll-assoc. virus 1+3 (samples: leaves1, petioles2, canes3)
GLRaV-2 Grapevine leafroll-assoc. virus 2 (samples: leaves1, petioles2, canes3)
GLRaV-3 Grapevine leafroll-assoc. virus 3 (samples: leaves1, petioles2, canes3)
GLRaV-4 strain 6 Grapevine leafroll-assoc. virus 4 strain 6 (samples: leaves1, petioles2, canes3)
GLRaV-4 strains Grapevine leafroll-assoc. virus generic 4 strains (samples: leaves1, petioles2, canes3)
GVA Grapevine virus A (samples: leaves1, petioles2, canes3)
GFkV Grapevine fleck virus (samples: leaves1, petioles2, canes3)
 
Our PCR Tests for Grapevine samples
GRBV Grapevine red blotch virus (samples: leaves1, petioles2, canes3)
GVA4 Grapevine virus A (samples: leaves1, petioles2, canes3)
GVB Grapevine virus B (samples: leaves1, petioles2, canes3)
RSPaV4 Rupestris stem pitting assoc. virus (samples: leaves1, petioles2, canes3)
FD Flavescence dorée phytoplasma (samples: leaves1, petioles2, canes3)
BN Bois noir phytoplasma (samples: leaves1, petioles2)
Service Offer
1 Leaves from young shoots early in the growing season.
2 Well-developed mature leaves, especially petioles and veins, from the lower part of the plant late in the growing season.
3 Phloem tissue from mature canes during dormancy
4 PCR tests of GVA and RSPaV are not in the range of accredited service
 
 
Our recommendations for sampling
 
Virus tests
We are employing efficient and reliable techniques for detection of viruses in grapevine.
However, the virus concentration varies considerably according to tissue source, virus and
physiological state of the host in function of time of the season. These facts have to be
considered for obtaining reliable results.
Basically, all viruses can be detected in mature canes during dormancy. When testing
leaves, however, viruses are separated into two distinct groups having each their specific
requirements. Under climatic conditions of Switzerland and Middle Europe, following
recommendations for tissue sampling can be given:
 
Virus Group A test: preferable tissue sampling
Leaves from young shoots and juicy bark early in the growing season as well as bark (phloem) scrapings from mature canes during dormancy.
 
Virus Group A test: unsuitable tissue sampling
Samples collected during the hot summer or old leaves in fall.
 
Virus Group B test: preferable tissue sampling
Well-developed mature leaves, especially petioles and veins, from the lower part of the plant late
in the season as well as bark (phloem) scrapings from mature canes during dormancy.
 
Virus Group B test: unsuitable tissue sampling
Young tissue early in the growing season.
 
For testing root stocks, wood of mature canes is the only reliable sample tissue.
 
Phytoplasma tests
Phytoplasma (Flavescence dorée, Bois noir) are best detectable in veins and petioles of
well-developed mature leaves late in the growing season, as for viruses of group B. During
the rest of the year, phytoplasma are preferably detected in root tissue.

Vegetable Tests


 
Our ELISA Tests for Vegetable samples
Aac Acidovorax avenae subsp. citrulli (e.g. watermelon, cucurbits)
ACMV African cassava mosaic virus (e.g. cassava) / NOTE: detected with the TYLCV reagent
BCMNV Bean common mosaic necrosis virus (e.g.bean)
BCMV Bean common mosaic virus (e.g. bean)
BGMV Bean golden mosaic virus (e.g. bean) / NOTE: detected with the TYLCV reagent
BNYVV Beet necrotic yellow vein virus (e.g. beet, spinach) 
CaMV Cauliflower mosaic virus (e.g. cabbage, cauliflower, broccoli, turnip, radish etc.)
CGMMV Cucumber green mottle mosaic virus (e.g. melon, watermelon, cucumber, pumpkin, squash, gourd, etc.)
CMV Cucumber mosaic virus (e.g. tomato, bean, celery, cucurbits, pepper, spinach, etc. )
CYSDV Cucumber yellow stunting disorder virus (e.g. cucurbits, etc.)
GCLV Garlic common latent virus (e.g. garlic, leek, and other Allium spp.)
ErLV Erysimum latent virus (various)
ICMV Indian cassava mosaic virus (e.g. cassava) / NOTE: detected with the TYLCV reagent
LMV Lettuce mosaic virus (e.g. lettuce, pea, spinach, etc.)
LYSV Leek yellow stripe virus (e.g. leek, rarely in otherAllium spp.)
MiLBVV Mirafiori lettuce big-vein virus (e.g. lettuce)
OYDV Onion yellow dwarf virus (e.g. garlic, leek, onion, shallot, and other Allium spp.)
PepMV Pepino mosaic virus (e.g. tomato)
PMMoV Pepper mild mottle virus (e.g. pepper)
Poty group Poty virus (various)
PRSV Papaya ringspot virus = Watermelon mosaic virus 1 (e.g. cucumber, squash, watermelon, other cucurbits, papaya, etc.)
PVX Potato virus X (e.g. potato, tomato)
PVY Potato virus Y (e.g. pepper, potato, tomato)
RaMV Radish mosaic virus (e.g. cabbage, arugula)
SLCV Squash leaf curl virus (e.g. bean, cucurbits) / NOTE: detected with the TYLCV reagent
SMV Soybean mosaic virus (e.g. soybean, field bean, bean, pea, lupine)
SqMV Squash mosaic virus (e.g. cucumber, squash, other cucurbits)
TBSV Tomato bushy stunt virus (e.g. tomato, pepper, artichokes)
TCSV Tomato chlorotic spot virus (e.g. tomato.) / NOTE: detected with the TSWV reagent
TMV Tobacco mosaic virus (e.g. beet, pepper, cucurbits, bean, tomato)
TNV Tobacco necrosis virus (e.g. bean, cucurbits, tomato)
ToANV Tomato apex necrosis virus = Tomato marchitez virus (e.g. tomato, tomatillo)
ToBRFV Tomato brown rugose fruit virus (e.g. tomato, pepper)
ToMarV Tomato marchitez virus (see ToANV)
ToMoV Tomato mottle virus (e.g. tomato, bean) / NOTE: detected with the TYLCV reagent
ToMV Tomato mosaic virus (e.g. tobacco, tomato, etc.)
ToRSV Tomato ringspot virus (e.g. cucumber, rhubarb)
TSV Tobacco streak virus (e.g. bean, pea, pepper, tomato, etc.)
TSWV Tomato spotted wilt virus (e.g. bean, cucurbits, lettuce, spinach, tomato, etc.)
TuMV Turnip mosaic virus (e.g. brussels sprout, cabbage, cauliflower, turnip, etc.)
TYLCV Tomato yellow leaf curl virus (e.g. tomato, bean, squash)
TYMV Turnip yellow mosaic virus (e.g. turnip, cabbage)
Verticillium Verticillium spp. (e.g. tomato, celery)
WMV-1 Watermelon mosaic virus 1 (see PRSV)
WMV-2 Watermelon mosaic virus 2 (e.g. cucumber, pumpkin, squash, watermelon, etc.)
ZYMV Zucchini yellow mosaic virus (e.g. cucumber, watermelon, zucchini, squash, etc.)
 
Our PCR Tests for Vegetable samples
Lso Candidatus Liberibacter solanacearum (e.g. carrot seeds)
SBR* Syndrome Basses Richesses / Candidatus Arsenophonus phytopathogenicus (e.g. sugar beet)
ToBRFV Tomato brown rugose fruit virus (e.g. tomato, pepper)
 
* Tests are not in the range of accredited service

Fruit Tree & Small Fruit Tests

 
 

 
Our ELISA Tests for Fruit Tree & Small Fruit samples
ACLSV Apple chlorotic leaf spot virus (e.g. apple, pear, plum/prune, cherry, peach, quince, apricot)
ApMV Apple mosaic virus (e.g. apple, hop, plum/prune. Prum: very rare)
ApP Apple proliferation phytoplasma apple
ArMV Arabis mosaic virus (e.g. cherry, peach, elderberry, hop, raspberry, strawberry)
ASGV Apple stem grooving virus = Citrus tatter leaf virus (e.g. apple)
ASPV Apple stem pitting virus = Pear vein yellows virus (e.g. apple, pear)
CLRV Cherry leaf roll virus (e.g. cherry, peach, walnut, elderberry)
CTLV Citrus tatter leaf virus = Apple stem grooving virus (e.g. citrus, orange)
CTV Citrus tristeza virus (e.g. citrus)
PDV Prune dwarf virus (e.g. cherry, peach, plum/prune)
PeAMV Petunia asteroid mosaic virus (e.g. cherry, plum)
PNRSV Prunus necrotic ringspot virus (e.g. cherry, plum/prune, apricot, peach, hop)
Poty group Poty virus (various)
PPV Plum pox virus = Sharka (e.g. plum, apricot, peach, P. mahaleb and other Prunus spp., sour cherry)
PRSV Papaya ringspot virus = Watermelon mosaic virus 1 (e.g. papaya)
PVYV Pear vein yellows virus (see ASPV)
RBDV Raspberry bushy dwarf virus (e.g. raspberry, black raspberry)
RpRSV Raspberry ringspot virus (e.g. cherry, plum/prune, raspberry, strawberry, redcurrant)
SLRSV Strawberry latent ringspot virus (e.g. plum/prune, peach, cherry, elderberry, raspberry, strawberry, blackberry, blackcurrant, redcurrant)
SMV Soybean mosaic virus (e.g. soybean, field bean, bean, pea, lupine)
SMYEPV Strawberry mild yellow edge potexvirus (e.g. strawberry)
ToRSV Tomato ringspot virus (e.g. apple, peach, cherry, plum/prune,raspberry)
TRSV Tobacco ringspot virus (e.g. cherry, blueberry, papaya)
TSV Tobacco streak virus (e.g. strawberry, raspberry red and black)
Verticillium Verticillium spp. (e.g. apple, rose, maple)
Xfr Xanthomonas fragariae (e.g. strawberry)
 
Our PCR Tests for Fruit Tree & Small Fruit samples
Phytoplasma general in leaf veins, petioles, or roots of pomaceous, stone and small fruits
PPV Plum pox virus (Sharka) in leaves of stone fruits (Prunus spp.)

Field Crop Tests

 

 
Our ELISA Tests for Field Crop samples
AMV Alfalfa mosaic virus (e.g. alfaalfa, bean, pea, potato, tomato, etc.)
BNYVV Beet necrotic yellow vein virus (e.g. beet, spinach, etc.)
BYDV-B Barley yellow dwarf virus-B (e.g. barley, oat, wheat, triticale, maize)
BYDV-F Barley yellow dwarf virus-F (e.g. barley, oat, wheat, triticale, maize)
BYDV-RPV Barley yellow dwarf virus-RPV (see CYDV-RPV)
CaMV Cauliflower mosaic virus (e.g. cabbage, cauliflower, broccoli, turnip, radish etc.)
CMV Cucumber mosaic virus (e.g. bean, clover, beet, cucurbits, potato, tobacco, etc.)
CYDV-RPV Cereal yellow dwarf virus-RPV (e.g. barley, oat, wheat, triticale, maize)
GRSV Groundnut ringspot virus (e.g. groundnut, peanut) / NOTE: detected with the TSWV reagent
MCMV Maize chlorotic mottle virus (e.g. maize, sorghum (foodder, grain and weed))
MDMV Maize dwarf mosaic virus (e.g. maize, sorghum (foodder, grain and weed))
PeAMV Petunia asteroid mosaic virus (e.g. hop, spinach)
Poty group poty virus (various)
PVY Potato virus Y (e.g. tobacco)
RaMV Radish mosaic virus (e.g. rape, mustard, cabbage, arugula etc.)
SCMV Sugarcane mosaic virus (e.g. sugarcane, occasionally sorghum, maize and other Gramineae)
TMV Tobacco mosaic virus (e.g. bean, beet, tomato, tobacco, etc.)
TNV Tobacco necrosis virus (e.g. bean, beet, cucurbits, potato, soybean, tomato, tobacco, etc.)
TSV Tobacco streak virus (e.g. alfalfa, bean, cotton, soybean, tomato, tobacco, etc.)
TSWV Tomato spotted wilt virus (e.g. cucurbits, peanut, potato, soybean, tobacco, etc.)
TYMV Turnip yellow mosaic virus (e.g. turnip, cabbage, mustard, rape)
 
Our PCR Tests for Field Crop samples
HLVd Hop latent viroid (e.g. hop, hemp)
SBR* Syndrome Basses Richesses / Candidatus Arsenophonus phytopathogenicus (e.g. sugar beet)
 
* Tests are not in the range of accredited service

Ornamental Tests


 

Our ELISA Tests for Ornamental samples

AMV Alfalfa mosaic virus (e.g. lupine, Petunia)
ApMV Apple mosaic virus (e.g. periwinkle, Petunia, rose, other Rosaceae spp)
ArMV Arabis mosaic virus (e.g. forsythia, narcissus, Petunia, Prunus spp., rose, etc.)
CbMV Calibrachoa mottle virus (e.g. Calibrachoa, Petunia)
CMV Cucumber mosaic virus (e.g. begonia, Chrysanthemum, geranium, gladiolus, Impatiens, lily, orchids, Petunia, etc.)
ErLV Erysimum latent virus (various)
INSV Impatiens necrotic spot virus (e.g. begonia, carnation, Chrysanthemum, dahlia, geranium, gladiolus, Impatiensnarcissus, orchids, Petunia, etc.)
PeAMV Petunia asteroid mosaic virus (e.g. Petunia)
PFBV Pelargonium flower break virus (e.g. geranium)
PLCV Pelargonium leaf curl virus (e.g. geranium)
PLPV Pelargonium line pattern virus (e.g. geranium)
PNRSV Prunus necrotic ringspot virus (e.g. Petunia, rose)
Poty group Poty virus (various)
PVA Potato virus A (e.g. Petunia)
PVX Potato virus X (e.g. Petunia)
PVY Potato virus Y dahlia, (e.g. Petunia)
Rs Ralstonia solanacearum (e.g. geranium)
SLRSV Strawberry latent ringspot virus (e.g. gladiolus, narcissus, rose)
TMV Tobacco mosaic virus (e.g. geranium, Petunia)
TNV Tobacco necrosis virus (e.g. tulip)
ToMV Tomato mosaic virus (e.g. Petunia)
ToRSV Tomato ringspot virus (e.g. Chrysanthemum, carnation, geranium, gladiolus, Impatiens, orchids, Petuniarose, etc.)
TRSV Tobacco ringspot virus (e.g. begonia, Chrysanthemum, geranium, gladiolus, Impatiens, lily, narcissus, Petunia, rose, etc.)
TSV Tobacco streak virus (e.g. dahlia, Impatiens, Petunia)
TSWV Tomato spotted wilt virus (e.g. begonia, carnation, Chrysanthemum, dahlia, geranium, gladiolus, Impatiensorchids, Petunia, etc.)
Verticillium Verticillium spp. (e.g. Chrysanthemum, dahlia, rose)
Xcp Xanthomonas campestris pv. pelargonii = Xanthomonas hortorum pv. pelargonii (e.g. geranium)
Xhp Xanthomonas hortorum pv. pelargonii Xanthomonas campestris pv. pelargonii (e.g. geranium)
 
Our PCR Tests for Ornamental samples
Psa* Pseudomonas sp. actinidiae  (e.g. geranium, Petunia)
 
* Tests are not in the range of accredited service