PCR, qPCR
About Strategic Alliance
BIOREBA AG, Switzerland, and Qualiplante SAS, France, are pleased to announce they have entered into a global strategic alliance to jointly offer to the market a wide range of products for plant pathogen diagnostic testing based on molecular biology.
For more information please click Press release BIOREBA & QUALIPLANTE strategic alliance_E.pdf
Benefits of PCR and qPCR tests powered by Qualiplante & BIOREBA:
- Ready-to-use sets
- Optimized for your application
- Validated (sensitivity, specificity, repeatability, reproducibility)
- Lot-to-lot consistent
- Certificate of Analysis (CoA) included
- One-step-reactions
- Positive and negative controls included
- Internal Control IC host gene or IC RNA included
- Long shelf life
- Multiplex with high specificity
For more information please click Product Catalog_Molecular Diagnostic.pdf
PCR methods used for BIOREBA PCR tests,
powered by Qualiplante and by BIOREBA
Classical PCR methods
End-Point PCR
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End-Point PCR is the “classical” PCR method, whereby the DNA is detected after completion of PCR amplification. The presence or absence of the corresponding DNA product is determined by gel electrophoresis using staining of separated DNA fragments with a fluorescent dye, and digital imaging densitometry to visualize DNA bands. |
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End-Point RT-PCR
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End-Point RT-PCR is the “classical” PCR method using RNA as starting material. In a onestep reaction first, the RNA is reverse transcribed (RT) into cDNA, then the amplified cDNA is detected after completion of PCR amplification. The presence or absence of the corresponding DNA product is determined by gel electrophoresis using staining of separated DNA fragments with a fluorescent dye, and digital imaging densitometry to visualize DNA bands. |
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Nested End-Point PCR
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Nested End-Point PCR is based on the “classical” End-Point PCR method. Nested End-Point PCR reaction involves two distinct sets of primers used in two successive PCR runs. In the second PCR, a target within the first PCR product is amplified. This method allows to reduce non-specific binding and in parallel to run more total cycles to increase the amplification. The presence or absence of the corresponding DNA product is determined by gel electrophoresis as in a normal End-Point PCR. |
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Two-Step End Point RT-PCR
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The Two-Step End-Point RT-PCR is based on the End-Point RT-PCR method using RNA as starting material. The difference is based on the fact that the overall reaction is split into two separate reaction steps (Two-Step). In a first step the RNA is reverse transcribed (RT) into cDNA. In a second step, the cDNA is amplified and analyzed as described above in “End-Point PCR”. |
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Real time PCR (qPCR) methods
Taq-Man® qPCR
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Taq-Man® qPCR is a PCR method, whereby the amplified DNA is measured in real-time after each amplification cycle using fluorescent Taq- Man® probes. Taq-Man® probes are oligonucleotides, specific to the target DNA sequence, that have a fluorescent probe attached to the 5’ end and a quencher to the 3’ end. During PCR amplification the fluorophore will be cleaved away and is decoupled from the quencher. This leads to a strong increase of fluorescence intensity, which is proportional to the specific target DNA amplification. The fluorescence is directly measured and quantified in the real-time thermocycler. Distinct fluorophores can be used to detect multiple targets (Multiplex). |
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Taq-Man® RT-qPCR
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Taq-Man® RT-qPCR uses RNA as starting material. In a one-step reaction, the RNA is reverse transcribed (RT) into cDNA, then the cDNA is amplified during the same reaction and the fluorescence of Taq-Man® probes is directly measured and quantified in the real-time thermocycler. |
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SYBR®-Green qPCR
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SYBR®-Green qPCR is a PCR method, whereby the amplified DNA is measured in real-time after each amplification cycle using the fluorescent dye SYBR®-Green. SYBR®-Green binds only to double-stranded DNA. When SYBR®-Green binds to the doublestranded DNA of the PCR products, it emits fluorescence. This leads to a strong increase in intensity of fluorescence, which is proportional to the specific target DNA amplification. Since SYBR®-Green is not able to bind to single-stranded DNA, the fluorescence signal fast and rapidly decreases during the DNA denaturation step which allows a melting curve analysis. |
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SYBR®-Green RT-qPCR
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SYBR®-Green RT-qPCR uses RNA as starting material. In a one-step reaction first, the RNA is reverse transcribed (RT) into cDNA, then the cDNA is amplified during the same reaction and the fluorescence of bound SYBR®-Green dye is directly measured and quantified in the real-time thermocycler. |
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Synonym PLRV: Polerovirus PLRV / Synonym PVY: Potyvirus yituberosi
Art. Nr.: 839210
Mengeneinheit: 1
Art. Nr.: 839210
Mengeneinheit: 1
Synonym PLRV: Polerovirus PLRV / Synonym PVY: Potyvirus yituberosi
Art. Nr.: 839225
Mengeneinheit: 1
Art. Nr.: 839225
Mengeneinheit: 1
Synonym PLRV: Polerovirus PLRV / Synonym PVY: Potyvirus yituberosi
Art. Nr.: 839610
Mengeneinheit: 1
Art. Nr.: 839610
Mengeneinheit: 1
Synonym PLRV: Polerovirus PLRV / Synonym PVY: Potyvirus yituberosi
Art. Nr.: 839625
Mengeneinheit: 1
Art. Nr.: 839625
Mengeneinheit: 1
Synonym PLRV: Polerovirus PLRV / Synonym PVY: Potyvirus yituberosi
Art. Nr.: 839200
Mengeneinheit: 1
Art. Nr.: 839200
Mengeneinheit: 1